Recombinant human Ferredoxin 1/FDX1 protein
Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Recombinant human Ferredoxin 1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Adrenodoxin, Adrenal ferredoxin, Hepatoredoxin, ADX, FDX, LOH11CR1D, Adrenodoxin, mitochondrial, FDX1, Ferredoxin 1
Expression System
E.coli
Antigen Species
Human
Tag
T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
15 kDa (139aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mandai T., et al. (2009) . FEBS J. 276 (8) :2416-29. ; ; Pichiorri F., et al. (2009) . J Biol Chem. 284 (2) :1040-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_004100.1
Uniprot Accession Number
P10109
Species
Human
AA Sequence
SSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS
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