Recombinant human Riboflavin kinase/RFP protein

Riboflavin kinase, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. It is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN) . Recombinant riboflavin kinase was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RIFK, RP11-422N19.2, RFK, 0610038L10Rik, AF031381, KOI 4, Riboflavin kinase, ATP:riboflavin 5' phosphotransferase, Flavokinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

20.5 kDa (182aa) confirmed by MALDI-TOF

Additionnal Information

Riboflavin kinase, RIFK, RP11-422N19.2, RFK, 0610038L10Rik, AF031381, KOI 4, Riboflavin kinase, ATP:riboflavin 5' phosphotransferase, Flavokinase, ATGP0358-10 µg, ATGP0358-20 µg, ATGP0358-50 µg, ATGP0358-100 µg, ATGP0358-250 µg, ATGP0358-500 µg, ATGP0358-1 mg, ATGP0358-10, ATGP0358-20, ATGP0358-50, ATGP0358-100, ATGP0358-250, ATGP0358-500, ATGP0358-1

References & Citations

Karthikeyan S, et al. (2003) Structure. 11 (3) :265-73.; ; Yazdanpanah B, et al. (2009) Nature. 460 (7259) :1159-63.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_060809

Uniprot Accession Number

Q969G6

Species

Human

AA Sequence

MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMN GH