Recombinant human PTEN protein
PTEN, also known as phosphatase and tensin homolog, is a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein acts as both a dual-specificity protein phosphatase and a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. Recombinant PTEN protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Phosphatase and tensin homolog, BZS, MHAM, MMAC1, PTEN1, TEP1, Phosphatase and tensin homolog, PTEN, PTEN1, mutated in multiple advanced cancers 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
49.3 kDa (423aa)
Additionnal Information
References & Citations
Li DM., et al. (1997) Cancer Res. 57 (11) :2124-9. ; ; Maehama T., et al. (1998) J Biol Chem. 273 (22) :13375-8. ;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 2mM DTT, 100 mM NaCl, and 20% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_000305.3
Uniprot Accession Number
P60484
Species
Human
AA Sequence
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