Recombinant human PTEN protein

PTEN, also known as phosphatase and tensin homolog, is a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein acts as both a dual-specificity protein phosphatase and a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. Recombinant PTEN protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Phosphatase and tensin homolog, BZS, MHAM, MMAC1, PTEN1, TEP1, Phosphatase and tensin homolog, PTEN, PTEN1, mutated in multiple advanced cancers 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

49.3 kDa (423aa)

Additionnal Information

PTEN, Phosphatase and tensin homolog, BZS, MHAM, MMAC1, PTEN1, TEP1, Phosphatase and tensin homolog, PTEN, PTEN1, mutated in multiple advanced cancers 1, ATGP0357-10 µg, ATGP0357-20 µg, ATGP0357-50 µg, ATGP0357-100 µg, ATGP0357-250 µg, ATGP0357-500 µg, ATGP0357-1 mg, ATGP0357-10, ATGP0357-20, ATGP0357-50, ATGP0357-100, ATGP0357-250, ATGP0357-500, ATGP0357-1

References & Citations

Li DM., et al. (1997) Cancer Res. 57 (11) :2124-9. ; ; Maehama T., et al. (1998) J Biol Chem. 273 (22) :13375-8. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM EDTA, 2mM DTT, 100 mM NaCl, and 20% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_000305.3

Uniprot Accession Number

P60484

Species

Human

AA Sequence

MTAIIKEIVS RNKRRYQEDG FDLDLTYIYP NIIAMGFPAE RLEGVYRNNI DDVVRFLDSK HKNHYKIYNL CAERHYDTAK FNCRVAQYPF EDHNPPQLEL IKPFCEDLDQ WLSEDDNHVA AIHCKAGKGR TGVMICAYLL HRGKFLKAQE ALDFYGEVRT RDKKGVTIPS QRRYVYYYSY LLKNHLDYRP VALLFHKMMF ETIPMFSGGT CNPQFVVCQL KVKIYSSNSG PTRREDKFMY FEFPQPLPVC GDIKVEFFHK QNKMLKKDKM FHFWVNTFFI PGPEETSEKV ENGSLCDQEI DSICSIERAD NDKEYLVLTL TKNDLDKANK DKANRYFSPN FKVKLYFTKT VEEPSNPEAS SSTSVTPDVS DNEPDHYRYS DTTDSDPENE PFDEDQHTQI TKV