Recombinant human PGK1 protein
Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGKA, MIG10
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
46.8 kDa (437aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Svaasand EK., et al. (2007) Muscle Nerve. 36 (5) :679-84. ; ; Valentin C., et al. (1998) Hum Mutat. 12 (4) :280-7.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000282.1
Uniprot Accession Number
P00558
Species
Human
AA Sequence
Frequently Asked Questions
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