Recombinant human Midkine protein
Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
MDK, MK, NEGF2, midkine (neurite growth-promoting factor 2), FLJ27379, Midkine
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.7 kDa (144aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mashima T., et al. (2009) . Oncogene. 28 (1) :9-19. ; ; Jin Z., et al. (2008) . Clin Cancer Res. 14 (16) :5033-42.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, and 20% glycerol
Scientific Category
Growth factors
NCBI Accession Number
NP_002382
Uniprot Accession Number
P21741
Species
Human
AA Sequence
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