Recombinant human IL-6 protein
Interleukin-6, as known as IL-6, is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is also a 'myokine, ' a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6. It is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. Recombinant interleukin-6 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Specifications
Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 0.8 ng/mL.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.8 ng/mL.
Molecular Weight
23.1 kDa (204aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Ishihara, et al. (2002) Cytokine Growth Factor Rev. 13 (4-5) :357-68. ; ; Vgontzas AN, et al. (2005) Neuroimmunomodulation.12, 131-40.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000591.1
Uniprot Accession Number
P05231
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
Phospho-c-ABL (Tyr245) Polyclonal Antibody, PE-Cy7 Conjugated
Lentiviral mouse Zfp276 shRNA (UAS) - Lentiviral mouse Zfp276 shRNA (UAS, RFP) plasmid
![Rabbit Monoclonal MARCO Antibody (PLK1) [DyLight 488]](https://cdn.genprice.uk/genprice/genprice4.png)