Recombinant human IL-6 protein

Interleukin-6, as known as IL-6, is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is also a 'myokine, ' a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6. It is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. Recombinant interleukin-6 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 0.8 ng/mL.

Product Name Alternative

Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.8 ng/mL.

Molecular Weight

23.1 kDa (204aa) confirmed by MALDI-TOF

Additionnal Information

VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGP0352-10 µg, ATGP0352-20 µg, ATGP0352-50 µg, ATGP0352-100 µg, ATGP0352-250 µg, ATGP0352-500 µg, ATGP0352-1 mg, ATGP0352-010, ATGP0352-020, ATGP0352-050, ATGP0352-100, ATGP0352-250, ATGP0352-500, ATGP0352-01M

References & Citations

Ishihara, et al. (2002) Cytokine Growth Factor Rev. 13 (4-5) :357-68. ; ; Vgontzas AN, et al. (2005) Neuroimmunomodulation.12, 131-40.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000591.1

Uniprot Accession Number

P05231

Species

Human

AA Sequence

VPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM