Recombinant human Glycogenin 1 protein

Glycogenin 1 is an enzyme involved in glycogen biosynthesis. Glycogenin 1 is important for the function of self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Also, it plays a role in regulating glycogen metabolism and the attainment of maximal glycogen levels in skeletal muscle. Recombinant human Glycogenin 1, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glycogenin, GYG1, GYG, Glycogenin1, GYG 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-T7-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.2 kDa (365aa) confirmed by MALDI-TOF

Additionnal Information

Glycogenin1, Glycogenin, GYG1, GYG, Glycogenin1, GYG 1., ATGP0349-10 µg, ATGP0349-20 µg, ATGP0349-50 µg, ATGP0349-100 µg, ATGP0349-250 µg, ATGP0349-500 µg, ATGP0349-1 mg, ATGP0349-10, ATGP0349-20, ATGP0349-50, ATGP0349-100, ATGP0349-250, ATGP0349-500, ATGP0349-1

References & Citations

Yamashita H., et al. (1999) J Biol Chem. 274 (42) :29897-904. ; ; Declercg JP., et al. (2001) J Mol Biol. 311 (4) :751-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

AAH00033

Uniprot Accession Number

P46976

Species

Human

AA Sequence

MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMTDQAFVT LTTNDAYAKG ALVLGSSLKQ HRTTRRLVVL ATPQVSDSMR KVLETVFDEV IMVDVLDSGD SAHLTLMKRP ELGVTLTKLH CWSLTQYSKC VFMDADTLVL ANIDDLFDRE ELSAAPDPGW PDCFNSGVFV YQPSVETYNQ LLHLASEQGS FDGGDQGILN TFFSSWATTD IRKHLPFIYN LSSISIYSYL PAFKVFGASA KVVHFLGRVK PWNYTYDPKT KSVKSEAHDP NMTHPEFLIL WWNIFTTNVL PLLQQFGLVK DTCSYVNVED VSGAISHLSL GEIPAMAQPF VSSEERKERW EQGQADYMGA DSFDNIKRKL DTYLQ