Recombinant human Glycogenin 1 protein
Glycogenin 1 is an enzyme involved in glycogen biosynthesis. Glycogenin 1 is important for the function of self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Also, it plays a role in regulating glycogen metabolism and the attainment of maximal glycogen levels in skeletal muscle. Recombinant human Glycogenin 1, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glycogenin, GYG1, GYG, Glycogenin1, GYG 1.
Expression System
E.coli
Antigen Species
Human
Tag
His-T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
41.2 kDa (365aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Yamashita H., et al. (1999) J Biol Chem. 274 (42) :29897-904. ; ; Declercg JP., et al. (2001) J Mol Biol. 311 (4) :751-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
AAH00033
Uniprot Accession Number
P46976
Species
Human
AA Sequence
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