Recombinant human SAR1A protein

SAR1a gene homolog 1, also known as SAR1A, belongs to the small GTPase superfamily. Sar1 is an essential component of COPII vesicle coats involved in export of cargo from the ER. The GTPase activity of Sar1 functions as a molecular switch to control protein-protein and protein-lipid interactions that direct vesicle budding from the ER. GDP-bound Sar1 interacts with the membrane-bound exchange factor Sec12 and exchanges its bound GDP for GTP. Recombinant human SAR1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

SARA 1, Sara, SAR1a gene homolog 1 COPII associated small GTPase, SAR1a gene homolog 1 (S. cerevisiae), SAR1A, SAR1 homolog A (S. cerevisiae), SAR1 gene homolog A, SAR 1, Masra 2, GTP binding protein SAR1a, COPII-associated small GTPase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.5 kDa (218aa) confirmed by MALDI-TOF

Additionnal Information

COPII-associated small GTPase, GTP binding protein SAR1a, Masra 2, Masra2, SAR 1, SAR1, SAR1 gene homolog A, SAR1 homolog A (S. cerevisiae), SAR1A, SAR1a gene homolog (S cerevisiae) 1, SAR1a gene homolog (S. cerevisiae) 1, SAR1a gene homolog 1, SAR1a gene homolog 1 (S cerevisiae), SAR1a gene homolog 1 (S. cerevisiae), SAR1a gene homolog 1 COPII associated small GTPase, Sara, SARA 1, SARA1, ATGP0347-10 µg, ATGP0347-20 µg, ATGP0347-50 µg, ATGP0347-100 µg, ATGP0347-250 µg, ATGP0347-500 µg, ATGP0347-1 mg, ATGP0347-010, ATGP0347-020, ATGP0347-050, ATGP0347-100, ATGP0347-250, ATGP0347-500, ATGP0347-01M

References & Citations

Jones B., et al. (2003) Nat Genet. 34 (1) :29-31. ; ; Bi X., et al. (2007) Dev Cell. 13 (5) :635-45. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_064535

Uniprot Accession Number

Q9NR31

Species

Human

AA Sequence

MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID