Recombinant human Glutathione S-transferase theta 1/GSTT1 protein

Glutathione S-transferase theta 1 (GSTT1) is one of the GSTs' four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e. g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Glutathione S-transferase theta 1, GSTT1, GST class-theta-1, Glutathione transferase T1-1, Glutathione S-transferase theta 1 EC 2.5.1.18, Glutathione S transferase 5, Glutathione S transferase theta 1, GST class theta 1, Glutathione transferase T1 1, GST 5 5, GST CL1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31.5 kDa (277aa) confirmed by MALDI-TOF

Additionnal Information

Glutathione S-transferase theta1, GSTT1, Glutathione S-transferase theta 1, Glutathione S-transferase theta 1, GSTT1, GST class-theta-1, Glutathione transferase T1-1, Glutathione S-transferase theta 1 EC 2.5.1.18, Glutathione S transferase 5, Glutathione S transferase theta 1, GST class theta 1, Glutathione transferase T1 1, GST 5 5, GST CL1, ATGP0346-10 µg, ATGP0346-20 µg, ATGP0346-50 µg, ATGP0346-100 µg, ATGP0346-250 µg, ATGP0346-500 µg, ATGP0346-1 mg, ATGP0346-10, ATGP0346-20, ATGP0346-50, ATGP0346-100, ATGP0346-250, ATGP0346-500, ATGP0346-1

References & Citations

Faramawy MM., et al. (2009) . J Crit Care. 24 (3) :e7-10.; ; Delpisheh A., et al. (2009) . Eur J Obstet Gynecol Reprod Biol. 143 (1) :38-42.;

Other References

Toyoda Y, et al. Spontaneous Production of Glutathione-Conjugated Forms of 1,2-Dichloropropane: Comparative Study on Metabolic Activation Processes of Dihaloalkanes Associated with Occupational Cholangiocarcinoma. (Oxid Med Cell Longev. 2017) {https://pubmed.ncbi.nlm.nih.gov/28555163/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH07065.1

Uniprot Accession Number

P30711

Species

Human

AA Sequence

MGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR