Recombinant human Glutathione S-transferase theta 1/GSTT1 protein
Glutathione S-transferase theta 1 (GSTT1) is one of the GSTs' four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e. g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
31.5 kDa (277aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Faramawy MM., et al. (2009) . J Crit Care. 24 (3) :e7-10.; ; Delpisheh A., et al. (2009) . Eur J Obstet Gynecol Reprod Biol. 143 (1) :38-42.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAH07065.1
Uniprot Accession Number
P30711
Species
Human
AA Sequence
Frequently Asked Questions
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