Recombinant human Carbonyl reductase 3/CBR3 protein

Carbonyl reductase 3 (CBR3) is one of several monomeric NADPH-dependent oxidoreductases. This protein catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. It also contains three exons spanning 11. 2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. Some studies suggest that it mediates 9-cis-retinoic acid-induced cytostatis and is a potential prognostic marker for oral malignancy. Recombinant human CBR3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Carbonyl reductase 3, CBR3, NADPH-dependent carbonyl reductase, Carbonyl reductase (NADPH) 3, EC 1.1.1.184, hCBR3, Carbonyl reductase (NADPH) 3 EC 1.1.1.184, NADPH dependent carbonyl reductase 3, SDR21C2, Short chain dehydrogenase/reductase family 21C member 2.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33 kDa (297aa) confirmed by MALDI-TOF

Additionnal Information

Carbonyl reductase3, Carbonyl reductase 3, CBR3, NADPH-dependent carbonyl reductase, Carbonyl reductase (NADPH) 3, EC 1.1.1.184, hCBR3, Carbonyl reductase (NADPH) 3 EC 1.1.1.184, NADPH dependent carbonyl reductase 3, SDR21C2, Short chain dehydrogenase/reductase family 21C member 2., ATGP0345-10 µg, ATGP0345-20 µg, ATGP0345-50 µg, ATGP0345-100 µg, ATGP0345-250 µg, ATGP0345-500 µg, ATGP0345-1 mg, ATGP0345-10, ATGP0345-20, ATGP0345-50, ATGP0345-100, ATGP0345-250, ATGP0345-500, ATGP0345-1

References & Citations

Ohkura-Hada S., et al. (2008) . Open Dent J. 2:78-88.; ; Miura T., et al. (2009) . Life Sci. 85 (7-8) :303-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_001227

Uniprot Accession Number

O75828

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET PVYLALLPPD ATEPQGQLVH DKVVQNW