Recombinant human Ataxin-3 protein
Ataxin 3 is also known as Machado-Joseph disease protein 1. Machado-Joseph disease is one of several hereditary autosomal dominant neurodegenerative disorders. This protein contains trinucleotide CAG repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. Ataxin 3 interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB) -binding protein, p300, and p300/CREB-binding protein-associated factor and inhibits transcription by these coactivators. Also, ataxin-3 is a proteasome-associated factor that mediates the degradation of ubiquitinated proteins. Recombinant human Ataxin-3 was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
42.4 kDa (370aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Doss Pepe EW., et al. (2003) . Mol Cell Biol. 23 (18) :6469-83. ; ; Paulson., et al. (1997) . Ann Neurol. 41 (4) :453-62.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 50 mM NaCl, 10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
AAH33711
Uniprot Accession Number
P54252
Species
Human
AA Sequence
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