Recombinant human Aurora A protein

Aurora Kinase A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora kinase A is highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also this protein plays a crucial role in tumourigenesis and is overexpressed in many kinds of cancers. Recombinant human Aurora-A fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Serine/threonine-protein kinase 6, Aurora-A, AIK, ARK1, AuRA, BTAK, STK15, STK6, Aurora kinase A, AuRKA, Serine/threonine-protein kinase 6 Aurora 2, Aurora A, aurora kinase A, IAK, Serine/threonine kinase 15.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

47.9 kDa (423aa)

Additionnal Information

Aurora A, Serine/threonine-protein kinase 6, Aurora-A, AIK, ARK1, AuRA, BTAK, STK15, STK6, Aurora kinase A, AuRKA, Serine/threonine-protein kinase 6 Aurora 2, Aurora A, aurora kinase A, IAK, Serine/threonine kinase 15., ATGP0343-10 µg, ATGP0343-20 µg, ATGP0343-50 µg, ATGP0343-100 µg, ATGP0343-250 µg, ATGP0343-500 µg, ATGP0343-1 mg, ATGP0343-10, ATGP0343-20, ATGP0343-50, ATGP0343-100, ATGP0343-250, ATGP0343-500, ATGP0343-1

References & Citations

Tchatchou S., et al. (2007) . Cancer lett. 247 (2) : 266-72; ; Chen SS., et al. (2002) . EMBO J. 21 (17) : 4491-9;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 100 mM NaCl, 0.1mM EDTA, 0.1mM EGTA, 0.1mM PMSF, 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_003591.2

Uniprot Accession Number

O14965

Species

Human

AA Sequence

MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS