Recombinant human SAP18 protein

SAP18, also known as Sin3A-associated protein, is component of the histone deacetylase complex that plays an important role in the regulation of eukaryotic gene expression. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. It also has been shown to play a key role in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. Recombinant SAP18 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sin3A-associated protein 18kDa, Sin3A-associated protein, 2HOR0202, SAP18P, SAP18, Sin3A-associated protein, 18kDa Histone deacetylase complex subunit SAP18, Sin3-associated polypeptide p18.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.7 kDa (173aa) confirmed by MALDI-TOF

Additionnal Information

SAP18, Sin3A-associated protein 18kDa, Sin3A-associated protein, 2HOR0202, SAP18P, SAP18, Sin3A-associated protein, 18kDa Histone deacetylase complex subunit SAP18, Sin3-associated polypeptide p18., ATGP0340-10 µg, ATGP0340-20 µg, ATGP0340-50 µg, ATGP0340-100 µg, ATGP0340-250 µg, ATGP0340-500 µg, ATGP0340-1 mg, ATGP0340-10, ATGP0340-20, ATGP0340-50, ATGP0340-100, ATGP0340-250, ATGP0340-500, ATGP0340-1

References & Citations

Zhang Y., et al. (1997) Cell. 89 (3) :357-64. ; ; Matyash A, et al. (2009) J Biol Chem. 284 (5) :3012-20.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_005861.2

Uniprot Accession Number

O00422

Species

Human

AA Sequence

MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY