Recombinant human BMP-14/GDF-5 protein

Growth differentiation factor 5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This protein plays a role in chondrogenesis and chondrocyte metabolism, tendon and ligament tissue formation, and bone repair. It also increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease. GDF-5, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Growth differentiation factor 5, Bone morphogenetic protein 14 (BMP-14), Cartilage-derived morphogenetic protein 1 (CDMP-1), Lipopolysaccharide-associated protein 4 (LAP-4; LPS-associated protein 4), Radotermin, BMP14, CDMP1, LAP4, OS5, SYNS2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.8 kDa (141aa) confirmed by MALDI-TOF

Additionnal Information

BMP 14, BMP14, BMP-14, Bone morphogenetic protein 14, Cartilage derived morphogenetic protein 1, Cartilage-derived morphogenetic protein 1, CDMP 1, CDMP1, CDMP-1, GDF 5, GDF5, GDF-5, Growth differentiation factor 5, LAP 4, LAP4, LAP-4, Lipopolysaccharide associated protein 4, Lipopolysaccharide-associated protein 4, LPS associated protein 4, LPS-associated protein 4, OS5, Radotermin, SYNS2, ATGP0334-10 µg, ATGP0334-20 µg, ATGP0334-50 µg, ATGP0334-100 µg, ATGP0334-250 µg, ATGP0334-500 µg, ATGP0334-1 mg, ATGP0334-010, ATGP0334-020, ATGP0334-050, ATGP0334-100, ATGP0334-250, ATGP0334-500, ATGP0334-01M