Recombinant human BMP-14/GDF-5 protein
Growth differentiation factor 5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This protein plays a role in chondrogenesis and chondrocyte metabolism, tendon and ligament tissue formation, and bone repair. It also increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease. GDF-5, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.8 kDa (141aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Feng G., et al. (2008) . Growth Factor. 26 (3) :132-42; ; Sullivan AM., et al. (2005) . J Anat. 207 (3) :219-26.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000548
Uniprot Accession Number
P43026
Species
Human
AA Sequence
MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R
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