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Recombinant human TBCA protein

Tubulin folding cofactor A, also known as TBCA, is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin in a quasi-native confirmation. This protein is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. Recombinant human TBCA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

12.8 kDa (108aa) confirmed by MALDI-TOF

Additionnal Information

TBCA, Tubulin folding cofactor A, Tubulin folding cofactor A, chaperonin cofactor a, tubulin specific chaperone a, TBCA, Tubulin folding cofactor A CFA, Co chaperonin associated with a & b tubulin, Cofactor A, TCP1 chaperonin cofactor A, Tubulin cofactor a., ATGP0333-10 µg, ATGP0333-20 µg, ATGP0333-50 µg, ATGP0333-100 µg, ATGP0333-250 µg, ATGP0333-500 µg, ATGP0333-1 mg, ATGP0333-10, ATGP0333-20, ATGP0333-50, ATGP0333-100, ATGP0333-250, ATGP0333-500, ATGP0333-1

References & Citations

Nolasco S., et al. (2005) . FEBS Lett. 579 (17) :3515-24; ; Tian G., et al. (1996) . Cell. 86 (2) :287-96;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_004598.1

Uniprot Accession Number

O75347

Species

Human

AA Sequence

MADPRVRQIK IKTGVVKRLV KEKVMYEKEA KQQEEKIEKM RAEDGENYDI KKQAEILQES RMMIPDCQRR LEAAYLDLQR ILENEKDLEE AEEYKEARLV LDSVKLEA

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