Recombinant human Troponin C2/TNNC2 protein

Troponin C, skeletal muscle, also known as TNNC2, is the central regulatory protein of striated muscle contraction, and modulates the Ca2+-activation characteristics of muscle fibers. Troponin has 3 subunits, Troponin I (Tn-1), Troponin T (Tn-T) and Troponin C (Tn-C) . Tn-I subunit inhibits actomyosin ATPase and Tn-T subunit binds tropomyosin and Tn-C, while Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. Mutations in all components of this complex have been associated with skeletal muscle disease. Recombinant human Troponin C was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Troponin C2, Troponin C1 slow, Troponin C type 2 (fast), Troponin C type 1 (slow), Troponin C skeletal muscle, Troponin C, TNNC2, TNNC, Slow skeletal and cardiac muscles, Skeletal troponin C, Skeletal muscle TNNC1, Fast skeletal type, Fast skeletal muscle troponin C, FAP85, CFAP85, Cardiac troponin C

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18.1 kDa (160aa) confirmed by MALDI-TOF

Additionnal Information

Troponin C (1160aa), Troponin C skeletal muscle, Troponin C, skeletal muscle, TNNC2, Troponin C, skeletal muscle TNNC1, Cardiac troponin C, TNNC, Troponin C1 slow, Fast skeletal muscle troponin C, Skeletal troponin C, Troponin C type 1 (slow), Troponin C type 2 (fast), Troponin C, slow skeletal and cardiac muscles, ATGP0332-10 µg, ATGP0332-20 µg, ATGP0332-50 µg, ATGP0332-100 µg, ATGP0332-250 µg, ATGP0332-500 µg, ATGP0332-1 mg, ATGP0332-10, ATGP0332-20, ATGP0332-50, ATGP0332-100, ATGP0332-250, ATGP0332-500, ATGP0332-1

References & Citations

Prentice H., et al. (1994) . J Mol Cell Cardial. 26 (10) :1393-401. ; ; Gahlmann R., et al. (1988) . J Mol Biol. 201 (2) :379-91. ; ; Li Y., et al. (2008) . Hereditas. 145 (6) :274-82.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 100 mM NaCl, 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_003270.1

Uniprot Accession Number

P02585

Species

Human

AA Sequence

MTDQQAEARS YLSEEMIAEF KAAFDMFDAD GGGDISVKEL GTVMRMLGQT PTKEELDAII EEVDEDGSGT IDFEEFLVMM VRQMKEDAKG KSEEELAECF RIFDRNADGY IDPEELAEIF RASGEHVTDE EIESLMKDGD KNNDGRIDFD EFLKMMEGVQ