Recombinant human Serpin B5/Maspin protein

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the respective carcinomas. Recombinant human Maspin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5, SERPINB5, PI5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor, clade B, member 5, Ovalbumin, PI-5, Serpin peptidase inhibitor, Serpin peptidase inhibitor, clade B (ovalbumin), member 5, SERPINB5, Spi5, Spi7.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

44.2 kDa (395aa) confirmed by MALDI-TOF

Additionnal Information

Serpin B5, Maspin, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, Serpin B5, Protease inhibitor 5, SERPINB5, PI5, Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5 Serine (or cysteine) peptidase inhibitor, clade B, member 5, Ovalbumin, PI-5, Serpin peptidase inhibitor, Serpin peptidase inhibitor, clade B (ovalbumin), member 5, SERPINB5, Spi5, Spi7., ATGP0331-10 µg, ATGP0331-20 µg, ATGP0331-50 µg, ATGP0331-100 µg, ATGP0331-250 µg, ATGP0331-500 µg, ATGP0331-1 mg, ATGP0331-10, ATGP0331-20, ATGP0331-50, ATGP0331-100, ATGP0331-250, ATGP0331-500, ATGP0331-1

References & Citations

Lockett J., et al. (2006) . J Cell Biochem. 97 (4) :651-60.; ; Khalkhali Ellis Z., et al. (2006) . Clin Cancer Res. 12 (24) :7279-83.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Tags & Cell Markers

NCBI Accession Number

AY893387

Uniprot Accession Number

P36952

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDALQLANSA FAVDLFKQLC EKEPLGNVLF SPICLSTSLS LAQVGAKGDT ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLIK RLYVDKSLNL STEFISSTKR PYAKELETVD FKDKLEETKG QINNSIKDLT DGHFENILAD NSVNDQTKIL VVNAAYFVGK WMKKFPESET KECPFRVNKT DTKPVQMMNM EATFCMGNID SINCKIMELP FQNKHLSMFI LLPKDVEDES TGLEKIEKQL NSESLSQWTN PSTMANAKVK LSIPKFKVEK MIDPKACLEN LGLKHIFSED TSDFSGMSET KGVALSNVIH KVCLEITEDG GDSIEVPGAR ILQHKDELNA DHPFIYIIRH NKTRNIIFFG KFCSP