Recombinant human WWOX protein

WWOX, also known as WW domain containing oxidoreductase, is a proapoptotic protein and a tumor suppressor protein. WWOX is found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This protein may function synergistically with TP53/p53 to control genotoxic stress-induced cell death. It also plays a role in tumor necrosis factor (TNF) -mediated cell death. Recombinant human WWOX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

WW domain containing oxidoreductase, WW domain containing oxidoreductase, D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WW domain containing oxidoreductase 5330426P09Rik, 9030416C10Rik, EC 1.1.1.- Fragile site FRA16D Oxireductase, MGC55975, PRO0128, Putative oxidoreductase, zgc:55975, Short chain dehydrogenase/reductase family 41C, member 1, WW domain-containing protein WWOX.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.3 kDa (254aa) confirmed by MALDI-TOF

Additionnal Information

WWOX, WW domain containing oxidoreductase, WW domain containing oxidoreductase, D16S432E, FOR, FRA16D, HHCMA56, PRO0128, SDR41C1, WOX1, WW domain containing oxidoreductase 5330426P09Rik, 9030416C10Rik, EC 1.1.1.- Fragile site FRA16D Oxireductase, MGC55975, PRO0128, Putative oxidoreductase, zgc:55975, Short chain dehydrogenase/reductase family 41C, member 1, WW domain-containing protein WWOX., ATGP0330-10 µg, ATGP0330-20 µg, ATGP0330-50 µg, ATGP0330-100 µg, ATGP0330-250 µg, ATGP0330-500 µg, ATGP0330-1 mg, ATGP0330-10, ATGP0330-20, ATGP0330-50, ATGP0330-100, ATGP0330-250, ATGP0330-500, ATGP0330-1

References & Citations

Bouteille N., et al. (2009), Oncogene. 28 (28) :2569-80.; ; Gourley C., et al. (2009) . Cancer Res. 69 (11) :4835-42.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

AAH03184.1

Uniprot Accession Number

Q9NZC7

Species

Human

AA Sequence

MAALRYAGLD DTDSEDELPP GWEERTTKDG WVYYANHTEE KTQWEHPKTG KRKRVAGDLP YGWEQETDEN GQVFFVDHIN KRTTYLDPRL AFTVDDNPTK PTTRQRYDGS TTAMEILQGR DFTGKVVVVT GANSGIGFET AKSFALHGAH VILACRNMAR ASEAVSRILE EWQQGAATTV YCAAVPELEG LGGMYFNNCC RCMPSPEAQS EETARTLWAL SERLIQERLG SQSG