Recombinant human Troponin T protein

Troponin T, also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy. Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Troponin T type 2 cardiac isoform 3, Troponin T type 2, cardiac isoform 3, CMH2, CMPD2, cTnT, RCM3, TnTC, Troponin T type 2, cardiac isoform 3 Cardiac muscle troponin T, MGC3889, TNNT 2, TNNT2, Cardiomyopathy hypertrophic 2, Cardiomyopathy dilated 1D (autosomal dominant), CMD1D, Troponin T cardiac muscle, Troponin T type 2 (cardiac), Troponin T type 2 cardiac, Troponin T2 cardiac.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.4 kDa (305aa) confirmed by MALDI-TOF

Additionnal Information

Troponin T, Troponin T type 2 cardiac isoform 3, Troponin T type 2, cardiac isoform 3, CMH2, CMPD2, cTnT, RCM3, TnTC, Troponin T type 2, cardiac isoform 3 Cardiac muscle troponin T, MGC3889, TNNT 2, TNNT2, Cardiomyopathy hypertrophic 2, Cardiomyopathy dilated 1D (autosomal dominant), CMD1D, Troponin T cardiac muscle, Troponin T type 2 (cardiac), Troponin T type 2 cardiac, Troponin T2 cardiac, ATGP0329-100, ATGP0329-10 µg, ATGP0329-20 µg, ATGP0329-50 µg, ATGP0329-100 µg, ATGP0329-250 µg, ATGP0329-500 µg, ATGP0329-1 mg, ATGP0329-10, ATGP0329-20, ATGP0329-50, ATGP0329-100, ATGP0329-250, ATGP0329-500, ATGP0329-1

References & Citations

Yu H., et al. (2009), Proteomics. 9 (3) :504-11.; ; Morita H., et al. (2008) . N Engl J Med. 358 (18) :1899-908.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.5M NaCl, 0.1mM PMSF, 1mM DTT, 100 mM imidazole

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_001001431.1

Uniprot Accession Number

P45379

Species

Human

AA Sequence

MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK