Recombinant human CITED2 protein
CITED2 is an important transcriptional cofactor involved in multiple organ development. It is a cAMP-responsive element-binding protein (CBP) /p300 interacting transcriptional modulator and a proposed negative regulator for hypoxia-inducible factor (HIF) -1alpha through its competitive binding with HIF-1alpha to CBP/p300. CITED2 is necessary for mouse fetal liver hematopoiesis and is required for the proper formation of the hyaloid vasculature and for lens morphogenesis. Recombinant CITED2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.5 kDa (278aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bijl J., et al. (2006) Blood. 108 (1) :116-22. ; ; Chen Y., et al. (2008) Development. 135 (17) :2939-48.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 50% glycerol, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006070.2
Uniprot Accession Number
Q99967
Species
Human
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