Recombinant human Carbonic Anhydrase 2/CA2 protein

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Product Name Alternative

Carbonic anhydrase II, CA-II, Carbonate dehydratase II, Carbonic anhydrase C, CAC, Cyanamide hydratase CA2, Car2

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 7,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Molecular Weight

29.2 kDa (260aa) confirmed by MALDI-TOF

Additionnal Information

Carbonic Anhydrase II, CA2, Carbonic anhydrase 2, CA-II, CAC, CA 2, CA II, CA2, CAI, Car 2, Car2, Carbonate dehydratase II, Carbonic anhydrase B, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase., ATGP0325-10 µg, ATGP0325-20 µg, ATGP0325-50 µg, ATGP0325-100 µg, ATGP0325-250 µg, ATGP0325-500 µg, ATGP0325-1 mg, ATGP0325-10, ATGP0325-20, ATGP0325-50, ATGP0325-100, ATGP0325-250, ATGP0325-500, ATGP0325-1

References & Citations

Hu PY., et al. (1992) . Hum Mutat. 1 (4) :288-92 ; ; Venta PJ., et al. (1991) . Am J Hum Genet. 49 (5) :1082-90;

Other References

Saito M, et al. A case of paraneoplastic optic neuropathy and outer retinitis positive for autoantibodies against collapsin response mediator protein-5, recoverin, and α-enolase. (BMC Ophthalmol. 2014) {https://pubmed.ncbi.nlm.nih.gov/24428923/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000058.1

Uniprot Accession Number

P00918

Species

Human

AA Sequence

MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK