Recombinant human BMF protein
Bcl2 modifying factor, also known as BMF belongs to the Bcl2 protein family of apoptosis mediators. Bmf is constitutively expressed in many tissues. This protein contains a single Bcl2 homology domain 3 (BH3), and has been shown to bind Bcl2 proteins and function as an apoptotic activator. Also, BMF is a key molecule for histone deacetylase (HDAC) inhibitors which alters the balance between acetylation and deacetylation, significantly increasing histone acetylation, while strongly inducing apoptosis in a variety of cancer cell types. Recombinant human BMF, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Bcl2 modifying factor isoform 3, Bcl2 modifying factor, isoform 3, BMF, Bcl2 modifying factor, isoform 3 Bcl 2 modifying factor, FLJ00065.
Expression System
E.coli
Antigen Species
Human
Tag
T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.6 kDa (144aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Morales AA., et al. (2004) . Leukemia. 18 (1) :41-7; ; Chen L., et al. (2005) . Mol Cell. 17 (3) :393-403;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing, 1mM DTT, 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_001003943
Uniprot Accession Number
Q96LC9
Species
Human
AA Sequence
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