Recombinant human Chromogranin A/CHGA protein

Chromogranin A is a member of the chromogranin/secretogranin (granins) family of neuroendocrine secretory proteins. Chromogranin A (CgA) represents the major soluble protein co-stored and co-released with catecholamines, within chromaffin cells secretory granules. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. It is considered a diagnostic and prognostic marker of several diseases, including a variety of tumours and cardiac heart failure. Recombinant human Chromogranin A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Cga, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin-2, EA-92, ES-43, Pancreastatin, parastatin, PHE5, PHES, SS-18, WA-8, WE-14, LF-19, Catestatin 2, SL21, AL-11, GV-19, GR-44, ER-37, GE-25, Serpinin-RRG, Serpinin, p-Glu serpinin precursor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

51.2 kDa (460aa) (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Chromogranin A, ATGP0323-10 µg, ATGP0323-20 µg, ATGP0323-50 µg, ATGP0323-100 µg, ATGP0323-250 µg, ATGP0323-500 µg, ATGP0323-1 mg, ATGP0323-10, ATGP0323-20, ATGP0323-50, ATGP0323-100, ATGP0323-250, ATGP0323-500, ATGP0323-1

References & Citations

Montero-Hadjadje M., et al. (2009) . J Biol Chem. 284 (18) :12420-31.; ; Hendy GN., et al. (1995) . Clin Invest Med. 18 (1) :47-65;

Other References

Carmon O, et al. Chromogranin A preferential interaction with Golgi phosphatidic acid induces membrane deformation and contributes to secretory granule biogenesis. (FASEB J. 2020) {https://pubmed.ncbi.nlm.nih.gov/32227388/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 0.1mM PMSF, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_001266.1

Uniprot Accession Number

P10645

Species

Human

AA Sequence

LPVNSPMNK GDTEVMKCIV EVISDTLSKP SPMPVSQECF ETLRGDERIL SILRHQNLLK ELQDLALQGA KERAHQQKKH SGFEDELSEV LENQSSQAEL KEAVEEPSSK DVMEKREDSK EAEKSGEATD GARPQALPEP MQESKAEGNN QAPGEEEEEE EEATNTHPPA SLPSQKYPGP QAEGDSEGLS QGLVDREKGL SAEPGWQAKR EEEEEEEEEA EAGEEAVPEE EGPTVVLNPH PSLGYKEIRK GESRSEALAV DGAGKPGAEE AQDPEGKGEQ EHSQQKEEEE EMAVVPQGLF RGGKSGELEQ EEERLSKEWE DSKRWSKMDQ LAKELTAEKR LEGQEEEEDN RDSSMKLSFR ARAYGFRGPG PQLRRGWRPS SREDSLEAGL PLQVRGYPEE KKEEEGSANR RPEDQELESL SAIEAELEKV AHQLQALRRG