Recombinant human Peroxiredoxin 3/PRDX3 protein

Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration. Recombinant human Peroxiredoxin 3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Product Name Alternative

Thioredoxin-dependent peroxide reductase mitochondrial, Thioredoxin-dependent peroxide reductase mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1), Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase, mitochondrial Antioxidant Protein 1, AOP1, MER5, PRX3, SP22, Peroxiredoxin-3

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Molecular Weight

21.5 kDa (195aa) confirmed by MALDI-TOF

Additionnal Information

Peroxiredoxin3, PRDX3, Thioredoxin-dependent peroxide reductase mitochondrial, Thioredoxin-dependent peroxide reductase mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1), Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase, mitochondrial Antioxidant Protein 1, AOP1, MER5, PRX3, SP22, Peroxiredoxin-3, ATGP0321-10 µg, ATGP0321-20 µg, ATGP0321-50 µg, ATGP0321-100 µg, ATGP0321-250 µg, ATGP0321-500 µg, ATGP0321-1 mg, ATGP0321-10, ATGP0321-20, ATGP0321-50, ATGP0321-100, ATGP0321-250, ATGP0321-500, ATGP0321-1

References & Citations

Cox AG., et al. (2009) . Biochemistry. 48 (27) :6495-501; ; Rhee SG., et al. (2005) . Free Radic Biol Med. 38 (12) :1543-52;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_006784

Uniprot Accession Number

P30048

Species

Human

AA Sequence

MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ