Recombinant human Nicotinamide N-Methyltransferase/NNMT protein
NNMT (Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Nicotinamide N-methyltransferase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
31.7 kDa (284aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Parsons RB., et al. (2002) . J Neuropathol Exp Neurol. 61 (2) :111-24.; ; Smith ML., et al. (1998) . Biochim Biophys Acta. 1442 (2-3) :238-44.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_006160
Uniprot Accession Number
P40261
Species
Human
AA Sequence
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