Recombinant human S100A4 protein
S100A4, also known as S100 calcium-binding protein A4, belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 gene have been implicated in tumor metastasis. Recombinant human S100A4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
13.8 kDa (121aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sherbet GV., et al. (2009) Cancer Lett. 280 (1) :15-30.; ; Ambartsumian N., et al. (1995) Gene. 159 (1) :125-30.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_002952.1
Uniprot Accession Number
P26447
Species
Human
AA Sequence
MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT DEAAFQKLMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K
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