Recombinant mouse IFN-beta 1/IFNB1 protein

Interferon beta 1, also known as IFN-beta, is produced mainly by fibroblasts and some epithelial cell types. IFN-beta is involved in the regulation of unspecific humoral immune responses and immune responses against viral infections. It also increases the expression of HLA class I antigens and blocks the expression of HLA class II antigens stimulated by IFN-gamma. Interferon beta 1 can be used for topic treatment of condylomata acuminata. Some studies suggest that Interferon beta 1 tends to prevent disease activity in patients with multiple sclerosis. Recombinant Interferon beta 1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ifb, IFN-beta, IFNB, Interferon beta 1, Interferon beta, Fibroblast interferon

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.0 kDa (182aa) confirmed by MALDI-TOF

Additionnal Information

IFN-b, Ifb, IFN-beta, IFNB, Interferon beta 1, ATGP0317-10 µg, ATGP0317-20 µg, ATGP0317-50 µg, ATGP0317-100 µg, ATGP0317-250 µg, ATGP0317-500 µg, ATGP0317-1 mg, ATGP0317-10, ATGP0317-20, ATGP0317-50, ATGP0317-100, ATGP0317-250, ATGP0317-500, ATGP0317-1

References & Citations

Fierlbeck G, et al. (1992) Hautarzt. 43 (1) :16-21. ; ; Panitch HS. (1992) Drugs. 44 (6) :946-62.;

Other References

Okumura F, et al. Activation of double-stranded RNA-activated protein kinase (PKR) by interferon-stimulated gene 15 (ISG15) modification down-regulates protein translation. (J Biol Chem. 2013) {https://www.ncbi.nlm.nih.gov/pubmed/23229543}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM HEPES buffer (pH 6.0) containing 0.5M NaCl, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_034640.1

Uniprot Accession Number

P01575

Species

Mouse

AA Sequence

INYKQLQLQ ERTNIRKCQE LLEQLNGKIN LTYRADFKIP MEMTEKMQKS YTAFAIQEML QNVFLVFRNN FSSTGWNETI VVRLLDELHQ QTVFLKTVLE EKQEERLTWE MSSTALHLKS YYWRVQRYLK LMKYNSYAWM VVRAEIFRNF LIIRRLTRNF QN