Recombinant human Aldo-keto Reductase 1B10/AKR1B10 protein

AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Aldo-keto reductase family 1 member B10, AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, AKR1B10, Aldose reductase like, Aldose reductase related protein, ARL 1, hARP, SI reductase, Small intestine reductase

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,500pmol/min/ug, and was obtained by measuring the decrease of NADP in absorbance at 340 nm resulting from NADPH at pH 7.0 at 37C.

Molecular Weight

36 kDa (316aa) confirmed by MALDI-TOF

Additionnal Information

AKR1B10, AKR1B11, AKR1B12, Aldo-keto reductase family 1, Aldo-keto reductase family 1 member B10, Aldo-keto Reductase1B10, Aldose reductase like, Aldose reductase related protein, ALDRLn, ARL 1, ARL1, ARL-1, hARP, HIS, member B10, member B10 Aldose reductase like, SI reductase, Small intestine reductase, ATGP0314-10 µg, ATGP0314-20 µg, ATGP0314-50 µg, ATGP0314-100 µg, ATGP0314-250 µg, ATGP0314-500 µg, ATGP0314-1 mg, ATGP0314-010, ATGP0314-020, ATGP0314-050, ATGP0314-100, ATGP0314-250, ATGP0314-500, ATGP0314-01M

References & Citations

Yabe D., et al. (1997) J Biol Chem. 272 (29) :18232-9.; ; Vorum H., et al. (1998) Biochim Biophys Acta. 1386 (1) :121-31.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_064695

Uniprot Accession Number

O60218

Species

Human

AA Sequence

MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY