Recombinant human Carbonyl reductase 1/CBR1 protein

CBR1 (Carbonyl reductase 1) is one of several monomeric NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. CBR1 is widely distributed in human tissues. CBR1 metabolizes many toxic environmental quinones and pharmacological relevant substrates such as the anticancer doxorubicin. CBR1 can also convert prostaglandin E2 to prostaglandin F2-alpha. Recombinant human CBR1 protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E (2) 9 reductase.

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

30 kDa (277aa) confirmed by MALDI-TOF

Additionnal Information

CBR1, Carbonyl reductase 1, Carbonyl reductase 1, CBR, hCBR1, SDR21C1, CBR1, Carbonyl reductase 1 15 hydroxyprostaglandin dehydrogenase [NADP+], Carbonyl reductase [NADPH] 1.CBR 1, CRN, NADPH dependent carbonyl reductase 1, Prostaglandin 9 ketoreductase, Prostaglandin E (2) 9 reductase., ATGP0311-10 µg, ATGP0311-20 µg, ATGP0311-50 µg, ATGP0311-100 µg, ATGP0311-250 µg, ATGP0311-500 µg, ATGP0311-1 mg, ATGP0311-10, ATGP0311-20, ATGP0311-50, ATGP0311-100, ATGP0311-250, ATGP0311-500, ATGP0311-1

References & Citations

Bateman RL., et al. (2008), J Biol Chem. 283 (51) :35756-62.; ; Plebuch M., et al. (2007) . Cancer Lett. 255 (1) :49-56.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_001748

Uniprot Accession Number

P16152

Species

Human

AA Sequence

MSSGIHVALV TGGNKGIGLA IVRDLCRLFS GDVVLTARDV TRGQAAVQQL QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLDVLVNN AGIAFKVADP TPFHIQAEVT MKTNFFGTRD VCTELLPLIK PQGRVVNVSS IMSVRALKSC SPELQQKFRS ETITEEELVG LMNKFVEDTK KGVHQKEGWP SSAYGVTKIG VTVLSRIHAR KLSEQRKGDK ILLNACCPGW VRTDMAGPKA TKSPEEGAET PVYLALLPPD AEGPHGQFVS EKRVEQW