Recombinant human 4-1BB Ligand/TNFSF9 protein

4-1BB ligand is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with 4-1BBL strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murine models of cancer, infectious disease and autoimmune disease. Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Human TNFSF9 is coated at 2 µg/mL (100 ul/well) can bind Human TNFRSF9 (CAT# ATGP3303) in a Functional ELISA assay.

Product Name Alternative

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, receptor 4-1BB ligand, homolog of mouse 4-1BB-L, CD137 ligand, CD137L

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human TNFRSF9 (CAT# ATGP3303) .

Molecular Weight

23.8 kDa (222aa) confirmed by MALDI-TOF

Additionnal Information

41BB Ligand, TNFSF9, Tumor necrosis factor ligand superfamily member 9, TNFSF9, 4-1BBL, CD137L, 4-1BB ligand, 4 1BB L, 4 1BB ligand, 4 1BBL, Cd137l, Cd157l, homolog of mouse 4 1BB L, homolog of mouse 4 1BBL, ILA ligand (TNF related), Ly63l, receptor 4 1BB ligand, Tumor necrosis factor (ligand) superfamily member 9., ATGP0308-10 µg, ATGP0308-20 µg, ATGP0308-50 µg, ATGP0308-100 µg, ATGP0308-250 µg, ATGP0308-500 µg, ATGP0308-1 mg, ATGP0308-10, ATGP0308-20, ATGP0308-50, ATGP0308-100, ATGP0308-250, ATGP0308-500, ATGP0308-1

References & Citations

Lee SW., et al. (2008) . Nat Immunol. 9 (8) :917-26; ; Sharma RK., et al. (2009) . Cancer Res. 69 (10) :4319-26;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_003802

Uniprot Accession Number

P41273

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE