Recombinant human Smad2 protein

SMAD2 (Mothers against decapentaplegic homolog 2) belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Phosphorylated Smad2 is then able to form a complex with Smad4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. Recombinant SMAD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sma- and Mad-related protein 2 isoform 1, Sma- and Mad-related protein 2 isoform 1, hMAD-2, hSMAD2, JV18, JV18-1, MADH2, MADR2, Sma- and Mad-related protein 2 isoform 1 JV181, MAD, MAD Related Protein 2, SMAD2.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

54.4 kDa (487aa)

Additionnal Information

Smad2, Sma- and Mad-related protein 2 isoform 1, Sma- and Mad-related protein 2 isoform 1, hMAD-2, hSMAD2, JV18, JV18-1, MADH2, MADR2, Sma- and Mad-related protein 2 isoform 1 JV181, MAD, MAD Related Protein 2, SMAD2., ATGP0307-10 µg, ATGP0307-20 µg, ATGP0307-50 µg, ATGP0307-100 µg, ATGP0307-250 µg, ATGP0307-500 µg, ATGP0307-1 mg, ATGP0307-10, ATGP0307-20, ATGP0307-50, ATGP0307-100, ATGP0307-250, ATGP0307-500, ATGP0307-1

References & Citations

Bonni., et al. (2001) Nat. Cell Biol. 3 (6) : 587-95.; ; Wotton, et al. (1999) Cell. 97 (1) : 29-39.;

Other References

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012) {https://pubmed.ncbi.nlm.nih.gov/22843993/}; ; Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005892.1

Uniprot Accession Number

Q15796

Species

Human

AA Sequence

MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPST CSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS