Recombinant human WIF-1 protein
Wnt inhibitory factor 1, also known as Wif-1, is a secreted protein that binds to Wnt proteins involved in the embryonic development and cancer, and inhibits their activity. Wif-1 protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF) -like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals. Recombinant human Wif-1 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Wnt inhibitory factor 1, Wnt inhibitory factor 1, WIF1, Wnt inhibitory factor 1
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.2 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
39.5 kDa (360aa)
Additionnal Information
References & Citations
Wissmann C. et al. (2003) J. Pathol. 201:204-12; ; Cebrat M. et al. (2004) Cancer Lett. 206:107-13;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 0.1mM PMSF, 1mM DTT
Scientific Category
Cancer
NCBI Accession Number
AAH18037.1
Uniprot Accession Number
Q9Y5W5
Species
Human
AA Sequence
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