Recombinant s.aureus Protein A protein
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Immunoglobulin G binding protein A, Immunoglobulin G binding protein A, Immunoglobulin G binding protein A Staphylococcal protein A, SPA
Expression System
E.coli
Antigen Species
S.aureus
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
48.1 kDa (434aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Guirnalda P., et al. (2007) Int J Parasitol. 37 (12) :1391-9.; ; Volpers C., et al. (2003) J Virol. 77 (3) :2093-104.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
ACD80064
Uniprot Accession Number
B3GGB2
Species
S.aureus
AA Sequence
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