Recombinant s.aureus Protein A protein

Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Immunoglobulin G binding protein A, Immunoglobulin G binding protein A, Immunoglobulin G binding protein A Staphylococcal protein A, SPA

Expression System

E.coli

Antigen Species

S.aureus

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.1 kDa (434aa) confirmed by MALDI-TOF

Additionnal Information

Protein A, Immunoglobulin G binding protein A, Immunoglobulin G binding protein A, Immunoglobulin G binding protein A Staphylococcal protein A, SPA, ATGP0304-10 µg, ATGP0304-20 µg, ATGP0304-50 µg, ATGP0304-100 µg, ATGP0304-250 µg, ATGP0304-500 µg, ATGP0304-1 mg, ATGP0304-10, ATGP0304-20, ATGP0304-50, ATGP0304-100, ATGP0304-250, ATGP0304-500, ATGP0304-1

References & Citations

Guirnalda P., et al. (2007) Int J Parasitol. 37 (12) :1391-9.; ; Volpers C., et al. (2003) J Virol. 77 (3) :2093-104.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

ACD80064

Uniprot Accession Number

B3GGB2

Species

S.aureus

AA Sequence

MAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DNNKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNKKPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQPA NHADANKAQA LPET