Recombinant human FKBP52/FKBP4 protein

FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. Recombinant human FKBP4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 700 nmol/min/mg, and is defined as the amount of cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

FK506 binding protein 4, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, HBI, Hsp 56, HSP binding immunophilin, p52, p59 protein, Peptidylprolyl cis trans isomerase, PPIase, Rotamase, T cell FK506 binding protein 59kD

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 700 nmol/min/mg, and is defined as the amount of cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

53.9 kDa (479aa) confirmed by MALDI-TOF

Additionnal Information

FKBP52, FKBP4, FK506 binding protein 4, FKBP52, FKBP59, HBI, Hsp56, p52, PPIase, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, FKBP4, FKBP52 protein, FKBP59, Hsp 56, HSP binding immunophilin, p59 protein, PPIase, Rotamase, Peptidyl prolyl cis trans isomerase, Peptidylprolyl cis trans isomerase, T cell FK506 binding protein 59kD., ATGP0303-10 µg, ATGP0303-20 µg, ATGP0303-50 µg, ATGP0303-100 µg, ATGP0303-250 µg, ATGP0303-500 µg, ATGP0303-1 mg, ATGP0303-10, ATGP0303-20, ATGP0303-50, ATGP0303-100, ATGP0303-250, ATGP0303-500, ATGP0303-1

References & Citations

Ostrow KL., et al. (2009) Clin Cancer Res. 15 (4) :1184-91; ; Ma D., et al. (2008) J Biol Chem. 283 (38) :25963-70;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_002005.1

Uniprot Accession Number

Q02790

Species

Human

AA Sequence

MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA