Recombinant aequorea victoria GFP protein
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Green fluorescent protein
Expression System
E.coli
Antigen Species
Aequorea victoria
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.8 kDa (238aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Millan MI, et al. (2009) . Medicina. 69 (3) :370-4.; ; Marshall J, et al. (1995) . Neuron, 14 (2) :211-5.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Fusion Tag & Detection
NCBI Accession Number
AAA27721.1
Uniprot Accession Number
P42212
Species
Aequorea victoria
AA Sequence
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