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Recombinant aequorea victoria GFP protein

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Green fluorescent protein

Expression System

E.coli

Antigen Species

Aequorea victoria

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.8 kDa (238aa) confirmed by MALDI-TOF

Additionnal Information

GFP, Green fluorescent protein, ATGP0302-10 µg, ATGP0302-20 µg, ATGP0302-50 µg, ATGP0302-100 µg, ATGP0302-250 µg, ATGP0302-500 µg, ATGP0302-1 mg, ATGP0302-10, ATGP0302-20, ATGP0302-50, ATGP0302-100, ATGP0302-250, ATGP0302-500, ATGP0302-1

References & Citations

Millan MI, et al. (2009) . Medicina. 69 (3) :370-4.; ; Marshall J, et al. (1995) . Neuron, 14 (2) :211-5.;

Other References

Jo J, et al. Cell-penetrating peptide (CPP) -conjugated proteins is an efficient tool for manipulation of human mesenchymal stromal cells. (Sci Rep. 2014) {https://www.ncbi.nlm.nih.gov/pubmed/24625570}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

AAA27721.1

Uniprot Accession Number

P42212

Species

Aequorea victoria

AA Sequence

MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
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