Recombinant human VAMP-5 protein
VAMP5, also known as vesicle-associated membrane protein 5, is a member of the synaptobrevin family and the SNARE superfamily. VAMP5 is the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. This protein may participate in a trafficking events that is associated with myogenesis, such as myoblast fusion and/or GLuT4 trafficking. Recombinant Vamp5 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
12.7 kDa (109aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zeng Q., et al. (1998) Mol Biol Cell. 9 (9) :2423-37.; ; Zeng Q., et al. (2003) J Biol Chem. 278 (25) :23046-54.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl, 0.5mM EDTA
Scientific Category
Neuroscience
NCBI Accession Number
NP_006625.1
Uniprot Accession Number
O95183
Species
Human
AA Sequence
MAG IELERCQQQA NEVTEIMRNN FGKVLERGVK LAELQQRSDQ LLDMSSTFNK TTQNLAQKKC WENIRYRIC
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