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Recombinant human VAMP-5 protein

VAMP5, also known as vesicle-associated membrane protein 5, is a member of the synaptobrevin family and the SNARE superfamily. VAMP5 is the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. This protein may participate in a trafficking events that is associated with myogenesis, such as myoblast fusion and/or GLuT4 trafficking. Recombinant Vamp5 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Vesicle-associated membrane protein 5, Vesicle-associated membrane protein 5, Myobrevin, Vesicle-associated membrane protein 5 Camp, VAMP 5, Vesicle associated membrane protein 5.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

12.7 kDa (109aa) confirmed by MALDI-TOF

Additionnal Information

VAMP5, Vesicle-associated membrane protein 5, Vesicle-associated membrane protein 5, Myobrevin, Vesicle-associated membrane protein 5 Camp, VAMP 5, Vesicle associated membrane protein 5., ATGP0298-10 µg, ATGP0298-20 µg, ATGP0298-50 µg, ATGP0298-100 µg, ATGP0298-250 µg, ATGP0298-500 µg, ATGP0298-1 mg, ATGP0298-10, ATGP0298-20, ATGP0298-50, ATGP0298-100, ATGP0298-250, ATGP0298-500, ATGP0298-1

References & Citations

Zeng Q., et al. (1998) Mol Biol Cell. 9 (9) :2423-37.; ; Zeng Q., et al. (2003) J Biol Chem. 278 (25) :23046-54.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 0.2M NaCl, 0.5mM EDTA

Scientific Category

Neuroscience

NCBI Accession Number

NP_006625.1

Uniprot Accession Number

O95183

Species

Human

AA Sequence

MAG IELERCQQQA NEVTEIMRNN FGKVLERGVK LAELQQRSDQ LLDMSSTFNK TTQNLAQKKC WENIRYRIC

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