Recombinant human ASF1A protein

Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146 Description ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

ASF1 anti-silencing function 1 homolog A, Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146, ASF1 anti-silencing function 1 homolog A Anti silencing function 1A, ASF1 anti silencing function 1 homolog A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

27 kDa (240aa) confirmed by MALDI-TOF

Additionnal Information

ASF1A, ASF1 anti-silencing function 1 homolog A, Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146, ASF1 anti-silencing function 1 homolog A Anti silencing function 1A, ASF1 anti silencing function 1 homolog A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, ATGP0296-10 µg, ATGP0296-20 µg, ATGP0296-50 µg, ATGP0296-100 µg, ATGP0296-250 µg, ATGP0296-500 µg, ATGP0296-1 mg, ATGP0296-10, ATGP0296-20, ATGP0296-50, ATGP0296-100, ATGP0296-250, ATGP0296-500, ATGP0296-1

References & Citations

Sanematsu F., et al. (2006) . J Biol Chem. 281 (19) :13817-27; ; Groth A., et al. (2005) . Mol Cell. 17 (2) :301-11;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT , 10% glycerol

Scientific Category

Histone Related

NCBI Accession Number

NP_054753.1

Uniprot Accession Number

Q9Y294

Species

Human

AA Sequence

MAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM