Recombinant human Annexin A1/ANXA1 protein

Annexin A1, also known as Lipocortin I, belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. It promotes membrane fusion and is also involved in exocytosis. Since Annexin A1 has phospholipase A2 inhibitory activity to bind from two to four calcium ions with high affinity, this protein may have potential anti-inflammatory activity. The detection of this protein by immunocytochemical means reportedly provides a simple, highly sensitive and specific assay for diagnosis of hairy cell leukemia. Recombinant human Annexin A1 protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, phospholipase A2 inhibitory protein, p35, Annexin Ac2-26, ANX1, LPC1

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.7 kDa (346aa) confirmed by MALDI-TOF

Additionnal Information

Phospholipase A2 inhibitory protein, p35, LPC-1, LPC1, LPC 1, Lipocortin-I, LipocortinI, Lipocortin-1, Lipocortin1, Lipocortin I, Lipocortin 1, HGNC:533, Chromobindin-9, Chromobindin9, Chromobindin 9, Calpactin-II, CalpactinII, Calpactin-2, Calpactin2, Calpactin II, Calpactin 2, ANXA1 protein, ANXA1, ANXA 1, ANX1, ANX A1, ANX 1, Annexin-I, AnnexinI, AnnexinA1, Annexin-1, Annexin1, Annexin I, Annexin Ac2-26, Annexin Ac2 26, Annexin A1, Annexin 1, ATGP0291-10 µg, ATGP0291-20 µg, ATGP0291-50 µg, ATGP0291-100 µg, ATGP0291-250 µg, ATGP0291-500 µg, ATGP0291-1 mg, ATGP0291-010, ATGP0291-020, ATGP0291-050, ATGP0291-100, ATGP0291-250, ATGP0291-500, ATGP0291-01M

References & Citations

Lim LH., et al. (2007) . FASEB J. 21 (4) :968-75; ; Kovacic RT., et al. (1991) . Biochemistry. 30 (37) :9015-21;

Other References

Jung SH et al., Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012) {https://pubmed.ncbi.nlm.nih.gov/22843993/}; ; Jung SH et al., Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH et al., Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}; ; Vais H et al., ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. (Elife. 2020) {https://pubmed.ncbi.nlm.nih.gov/32420875/}; ; Sakamoto N et al., Anti-annexin A4 antibody as a biomarker for desquamative interstitial pneumonia. (J Autoimmun. 2025) {https://pubmed.ncbi.nlm.nih.gov/40156961/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1mM DTT, 10% glycerol

Scientific Category

Serology

NCBI Accession Number

NP_000691.1

Uniprot Accession Number

P04083

Species

Human

AA Sequence

MAMVSEFLKQ AWFIENEEQE YVQTVKSSKG GPGSAVSPYP TFNPSSDVAA LHKAIMVKGV DEATIIDILT KRNNAQRQQI KAAYLQETGK PLDETLKKAL TGHLEEVVLA LLKTPAQFDA DELRAAMKGL GTDEDTLIEI LASRTNKEIR DINRVYREEL KRDLAKDITS DTSGDFRNAL LSLAKGDRSE DFGVNEDLAD SDARALYEAG ERRKGTDVNV FNTILTTRSY PQLRRVFQKY TKYSKHDMNK VLDLELKGDI EKCLTAIVKC ATSKPAFFAE KLHQAMKGVG TRHKALIRIM VSRSEIDMND IKAFYQKMYG ISLCQAILDE TKGDYEKILV ALCGGN