Recombinant human Sepiapterin reductase/SPR protein
Sepiapterin reductase (SPR) belongs to the short-chain dehydrogenase/reductase (SDR) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. SPR is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in SPR cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant human SPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
SPR, Sepiapterin reductase, SDR38C1, Short chain dehydrogenase/reductase family 38C, member 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.2 kDa (281aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Tobin JE., et al. (2007) . Brain Res. 30:42-7.; ; Ohye T., et al. (1998) . Biochem Biophys Res Commun. 251 (2) :597-602.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_003115
Uniprot Accession Number
P35270
Species
Human
AA Sequence
Frequently Asked Questions
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