Recombinant human SPG21 protein

Spastic paraplegia 21 (SPG21), also known as Maspardin, binds to the hydrophobic C-terminal amino acids of CD4 and may play a role as a negative regulatory factor in CD4-depenent T-cell activation. This protein is widely expressed in various tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in SPG21 cause Mast syndrome, an autosomal-recessive complicated form of hereditary spastic paraplegia characterized by dementia, thin corpus callosum and white matter abnormalities. Recombinant human SPG21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Spastic paraplegia 21 isoform a, ACP33, BM-019, GL010, Maspardin, MAST, Spastic paraplegia 21 isoform a Acid cluster protein 33, BM019, Spastic paraplegia 21 autosomal recessive Mast syndrome protein, SPG21.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.1 kDa (328aa) confirmed by MALDI-TOF

Additionnal Information

SPG21, Spastic paraplegia 21 isoform a, ACP33, BM-019, GL010, Maspardin, MAST, Spastic paraplegia 21 isoform a Acid cluster protein 33, BM019, Spastic paraplegia 21 autosomal recessive Mast syndrome protein, SPG21., ATGP0287-10 µg, ATGP0287-20 µg, ATGP0287-50 µg, ATGP0287-100 µg, ATGP0287-250 µg, ATGP0287-500 µg, ATGP0287-1 mg, ATGP0287-10, ATGP0287-20, ATGP0287-50, ATGP0287-100, ATGP0287-250, ATGP0287-500, ATGP0287-1

References & Citations

Simpson MA., et al. (2003) . Am J Hum Genet. 73 (5) :1147-56.; ; Zeitlmann L., et al. (2001) . J Biol Chem. 276 (12) :9123-32.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0)

Scientific Category

Neuroscience

NCBI Accession Number

NP_057714

Uniprot Accession Number

Q9NZD8

Species

Human

AA Sequence

MGEIKVSPDY NWFRGTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF RQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE YTHKSPRVHS LILCNSFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSMVS AEELEVQKGS LGISQEEQ