Recombinant human GRB2 protein

Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway. GRB2 is widely expressed and also binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Grb2 is composed of an SH2 domain flanked on each side by an SH3 domain. Inhibition of Grb2 function impairs developmental processes in various organisms and blocks transformation and proliferation of various cell types. Recombinant human GRB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Growth factor receptor-bound protein 2 isoform 1, Growth factor receptor-bound protein 2 isoform 1, ASH, EGFRBP-GRB2, GRB3-3, Growth factor receptor-bound protein 2 isoform 1 Abundant SRC homology, Adapter protein GRB2, Ash protein, EGFRBP GRB2, Epidermal growth factor receptor binding protein, Epidermal growth factor receptor binding protein GRB2, GRB 2, GRB2 adapter protein, Grb3 3, Growth factor receptor bound protein 2, Growth factor receptor bound protein 3, HT027, MST084, MSTP084, OTTHuMP00000166096, OTTHuMP00000166097, OTTHuMP00000166098, Protein ASH, SEM5, SH2/SH3 adapter GRB2.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

27 kDa (237aa) confirmed by MALDI-TOF

Additionnal Information

GRB2, Growth factor receptor-bound protein 2 isoform 1, Growth factor receptor-bound protein 2 isoform 1, ASH, EGFRBP-GRB2, GRB3-3, Growth factor receptor-bound protein 2 isoform 1 Abundant SRC homology, Adapter protein GRB2, Ash protein, EGFRBP GRB2, Epidermal growth factor receptor binding protein, Epidermal growth factor receptor binding protein GRB2, GRB 2, GRB2 adapter protein, Grb3 3, Growth factor receptor bound protein 2, Growth factor receptor bound protein 3, HT027, MST084, MSTP084, OTTHuMP00000166096, OTTHuMP00000166097, OTTHuMP00000166098, Protein ASH, SEM5, SH2/SH3 adapter GRB2., ATGP0286-10 µg, ATGP0286-20 µg, ATGP0286-50 µg, ATGP0286-100 µg, ATGP0286-250 µg, ATGP0286-500 µg, ATGP0286-1 mg, ATGP0286-10, ATGP0286-20, ATGP0286-50, ATGP0286-100, ATGP0286-250, ATGP0286-500, ATGP0286-1

References & Citations

Rudd CE., et al. (2009) Immunol Rev. 229 (1) :12-26.; ; Weisswange I., et al. (2009) Nature. 458 (7234) :87-91.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002077.1

Uniprot Accession Number

P62993

Species

Human

AA Sequence

MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG QTGMFPRNYV TPVNRNV