Recombinant human FUS2/NAA80 protein

N-acetyltransferase, also known as FuS2 (NAT6), is an enzyme that catalyzes the transfer of acetyl groups from acetyl-CoA to acrylamines. This enzyme is physically localized in the cytoplasm and its activity has been documented by its feasibility to acetylate the N-terminus of proteins using a ping-pong-like mechanism and by its substrate specificity. Since the Fus-2 gene maps to the chromosomal region 3p21. 3, which contains at least one tumor suppressor gene, the N-acetyltransferase functions of Fus-2 may be relevant to its potential role in cancer. Recombinant human N-acetyltransferase 6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Protein fus 2, Protein fusion-2, FuS-2, NAT6, N acetyltransferase 6

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

35.9 kDa (328aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Protein fusion-2, Protein fusion 2, Protein fusion2, Protein fus 2, NatH catalytic subunit, NAT6, N-alpha-acetyltransferase 80, NAA80, N acetyltransferase 6, FuS-2, FuS2, FuS 2, ATGP0285-10 µg, ATGP0285-20 µg, ATGP0285-50 µg, ATGP0285-100 µg, ATGP0285-250 µg, ATGP0285-500 µg, ATGP0285-1 mg, ATGP0285-010, ATGP0285-020, ATGP0285-050, ATGP0285-100, ATGP0285-250, ATGP0285-500, ATGP0285-01M

References & Citations

Zegerman P., et al. (2000) . Oncogene. 19 (1) :161-3.; ; Gatphayak K., et al. (2004) . Gene. 337:105-11;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 5.0) containing 150 mM NaCl, 30% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_036323

Uniprot Accession Number

Q93015

Species

Human

AA Sequence

MQELTLSPGP AKLTPTLDPT HRMELILSTS PAELTLDPAC QPKLPLDSTC QPEMTFNPGP TELTLDPEHQ PEETPAPSLA ELTLEPVHRR PELLDACADL INDQWPRSRT SRLHSLGQSS DAFPLCLMLL SPHPTLEAAP VVVGHARLSR VLNQPQSLLV ETVVVARALR GRGFGRRLME GLEVFARARG FRKLHLTTHD QVHFYTHLGY QLGEPVQGLV FTSRRLPATL LNAFPTAPSP RPPRKAPNLT AQAAPRGPKG PPLPPPPPLP ECLTISPPVP SGPPSKSLLE TQYQNVRGRP IFWMEKDI