Recombinant E.coli dsbE protein
DsbE, also called CcmG, is a reducing Dsb protein implicated in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. Its postulated function is to reduce disulphide bonds formed between correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH. Recombinant DsbE protein was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
E.coli
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18.1 kDa (161aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Reid E., et al. (2001) . Biochem J. 355 (1) :51-8.; ; Li Q., et al. (2001) . Biol Chem. 382 (12) :1679-86;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_416699
Uniprot Accession Number
P0AA86
Species
E.coli
AA Sequence
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